Molecular identification of intracellular survival related Brucella melitensis virulence factors

Introduction and Aim: Brucellosis is an important zoonotic disease caused by Brucella spp. which intracellular gram-negative bacterium. melitensis lacks the "traditional" virulence factors such as exotoxins or cytolysins, but capable of persisting intracellularly evading immune system. This study aims to identify B. using PCR discover genes associated with its severity for early detection therapy. Materials Methods: Ten ml unclotted blood sample was collected from each patient (n=100) suspected be infected brucellosis. The Castaneda technique used inoculate samples onto Basel agar a selective supplement tryptone soy broth in diphasic flask. Biochemical tests were identifying isolated colonies. isolates further confirmed polymerase chain reaction (PCR) using, primers targeting specific region (IS711 gene) genome. Multiplex determine four related (lps B, mgtA, omp25, CBG) all positive samples. Results: detected 9% (9/100) Among factors, LpsB while, omp25 CBG 66.6% 55.5% isolates, respectively. Conclusion: could diagnosed rapidly molecular techniques. also lpsB, CBG, that are crucial bacterium's pathogenicity environment.